REPRODUCTION

Lecture Notes

The primary function of sex organs are to form gametes (haploid sex cells like sperm and oocytes). A typical body cell has 23 pairs of chromosomes containing all of the genes that code for all proteins. A haploid cell has 1/2 of the number of chromosomes (or 23) which, during fertilization, fuses with 23 from other gender's haploid sex cell to form a new individual.
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I. Male reproductive anatomy

A. Scrotum 1. Skin sac holding paired testes provides protection against physical damage and infection.

2. Provides thermoregulation as high temp inhibits spermatogenesis
B. Testes 1. contain hundreds of seminiferous tubules which make sperm.

2. endocrine cells between tubules ( interstitial cells or cells of Leydig) secrete testosterone
C. Ducts 1. Epididymis -site of storage and maturation of sperm

2. Ductus ( or vas) deferens -pathway for sperm to continue (cut during vasectomy)

3. Ejactulatory duct
receives secretions of seminal vesicle and prostate glands
stores sperm to be ejaculated into urethra

4. Urethra -pathway for sperm and semen out of the body.

5. Penis -male copulatory organ contains erectile columns for erection

a. Corpus spongiosa (anterior) contains urethra

b. Copora cavernosum (posterior)
 
 
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D. Semen 1. combined secretions and sperm

2. seminal vesicle -most volume, alkaline (to neutralize acidic secretions of female) and high fructose (to provide nutrition for sperm)

3. prostate -promotes sperm activity (for instance, promotes movement)

List sequence that a sperm must travel from site of production to outside the body. Describe functions of each organ the sperm must pass through.
II. Male reproductive physiology A. Spermatogenesis -a sequence of processes continuous since puberty resulting in 300 million sperm/day 1. Processes needed a. Mitosis
cell division process that involves exact duplication of nuclear and cytoplasmic materials into two cells, each with normal amount (body cells have 2 pairs each of 23 chromosomes, also known as 2N or diploid cells) of genetic information . One division results in replacement of spermatogonia stem cells to maintain their reserve.

b. Meiosis
cell division process that involves NOT exact duplication of nuclear and cytoplasmic materials into four cells, each with one half (N or haploid ) or normal amount of genetic information. Two divisions result in halving the amount of genetic information to make the haploid cell (primary spermatocyte to secondary spermatocyte, secondary spermatocyte to spermatid )

c. Differentiation
biochemical change in a cell to make it a different cell results in formation of spermatozoa (or sperm) into spermatid

2. Cells needed a. Spermatogenic cells
family of cells to become sperm (spermatogonia through spermatozoa)

b. Sustentacular cells
nourish spermatogenic cells
form blood/testis barrier to keep sperm from immune system

c. Interstitial cells ( cells of Leydig)
produce testosterone
3. Specific sequence for spermatogenesis

a. Spermatogonia are replaced via mitosis-maintains reserve of these stem cells.

b. Spermatogonia differentiate into primary spermatocytes

c. Each primary spermatocyte begins first division of meiosis- to make two secondary spermatocytes

d. Each secondary spermatocytes undergo second meiotic division to make four spermatids

e. Spermatids differentiate into sperm

Therefore, production of four sperm from one primary spermatocyte
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Describe the processes, including important cell functions, that must occur for sperm to be produced. .

B. Control of male reproduction via negative feedback of testosterone levels 1. Hypothalamus
If testosterone levels are low then hypothalamus releases Gonadotropin releasing hormone (GnRH)

2. Increased GnRH causes anterior pituitary gland to release gonadotropins

a. Follicle Stimulating Hormone (FSH)
which causes sustentacular cells to increase nutrition and spermatogenesis

b. Leutinizing hormone, or Interstitial Cell Stimulating Hormone (LH or ICSH), gonadotropin
targets interstitial cells which then produce more testosterone
 

3. Increased testosterone
increases rate of spermatogenesis at seminiferous tubules
maintains secondary sex characteristics of male body
inhibits GnRH production at hypothalamus

4. less GnRH inhibits LH production and therefore testosterone production
(classic example of negative feedback mechanism via hypothalamic releasing hormones)

Describe negative feedback loop that regulates testosterone production. Describe functions of Testosterone. .

III. Female reproductive anatomy


A. Ovaries

1. oogenic cells
examples-oogonia, primary oocyte, secondary oocyte
secondary oocyte is gamete cell that is released (ovulated) into uterine tube

2. Follicular cells-surround a single oocyte cell to form a "follicle'
produce estrogen and progesterone

B. Uterine tube 1. End near ovary (fimbriae) suction up secondary oocyte (if not, then ectopic pregnancies) to transfer them towards uterus
2. Site of fertilization

C. Uterus
1. Perimetrium -serous membrane (visceral peritoneum) for lubrication to reduce friction between abdominopelvic organs.

2. Myometrium -smooth muscle tissue for birth contractions.
Readied by increasing estrogen during pregnancy but controlled by Oxytocin

3. Endometrium -epithelial secretory tissue for nutritional support of embryo via its blood supply
controlled by estrogen and progesterone

D. Vagina
female copulatory organ and birth canal
highly acidic to decrease microbial (and sperm) survival

E. External genitalia

1. Labia majora and minora

2. Clitoris

3. Mons pubis/pubic symphysis
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List sequence that a sperm must travel from its site of production to site of fertilization of secondary oocyte. Describe functions of each organ the sperm must pass through.

F. Breasts -modified sweat glands embedded in adipose tissue.
1. Mammary glands form milk.
Readied by increasing estrogen during pregnancy but controlled by Prolactin.

2. Myoepithelial cells surrounding glands and mammary ducts eject milk>
Readied by increasing estrogen during pregnancy but controlled by oxytocin.

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IV. Physiology of female reproduction

A. Oogenesis
a sequence of discontinuous processes resulting in one secondary oocyte ovulated every month


1. Processes required.
Mitosis
Meiosis


2. Cells required
oogenic cells (oogonia, primary oocyte, secondary oocyte)
ovarian follicular cells (produce estrogen and progesterone)

3. Specific process

a) Prior to birth
1. oogonia have undergone mitosis to create reserve of oogonia (stem) cells.
2. oogonia then differentiate to about 700,000 primary oocytes which are surrounded by thin layer of follicular cells (gamete's age is woman's age).

b. Every monthly reproductive cycle from puberty to menopause

1. primary oocytes undergo first of meiotic divisions to become secondary oocyte.
meiotic division in females is NOT equal with one cell -the oocyte- being large and the other-the polar body- being small and often dying.
increasing number of follicle cells continues to produce more estrogen

2. secondary oocyte is ovulated (in response to LH surge) and captured by fimbriae
(mature follicle bursts).


3 . Fate of oocyte

If NOT fertilized then oocyte lost and cycle begins again.
If fertilized then embryonic development begins (zygote results)
B. Hormonal control of female reproduction

1. GnRH
Low levels of Estrogen and Progesterone increase GnRH.
Increased GnRH triggers release of FSH at beginning of reproductive cycle.


2. FSH
Increased FSH initiates the growth of follicles at the beginning of each menstrual cycle promoting increased levels of Estrogen

3. Estrogen
a) Increasing estrogen levels promote production of LH (stored in anterior pituitary until mid-cycle); initiates growth of endometrium, maintain female secondary sex characteristics
b) very high levels trigger release of LH (mid cycle).


4. LH
Increased LH causes rupture of mature follicle (ovulation) and formation of corpus luteum which produces increased amounts of progesterone and, to lesser degree, estrogen.

Increasing estrogen and progesterone inhibit LH and FSH production (late cycle) to prevent second ovulation.

5 . Progesterone
a ) increased levels primarily maintain endometrium for implantation (promote gestation by maintaining endometrial nutrient supply)
If oocyte is fertilized then embryonic development begins (embryo will produce hormone to maintain corpus luteum)
If oocyte NOT fertilized then corpus luteum dies and progesterone levels drop. Decreased levels, along with lower levels of Estrogen, trigger destruction of endometrium (menses).

Describe feedback loops that control estrogen and progesterone levels. Describe the function of GnRH, LH, FSH, Estrogen and Progesterone..

C.Reproductive phases 1. Menstrual phase (days 1-5)
a) Ovary
primary oocyte inactive
proliferation of follicular cells under influence of FSH. Follicles begin to grow from primary to secondary (not mature) follicles.
b) Uterus
Endometrium is shedding (the period)

2. Preovulatory phase (days 6-13)
a) Ovary
primary oocyte finishes second meiotic step to become secondary oocyte
continued proliferation of follicular cells. Follicles grow from secondary to mature follicles. (Estrogen levels rising)
b) Uterus
Endometrium is proliferating under influence of Estrogen

3. Ovulation (day 14)
a) Ovary
secondary oocyte is released, stimulated by LH peak, as mature follicle bursts
burst mature follicle seals and begins to form corpus luteum
b) Uterus
Endometrium continues to proliferate

3. Postovulatory phase (days 15-28)
a) Ovary and Uterine tube
secondary oocyte begins travel to uterus down uterine tube
ruptured follicle seals and becomes corpus luteum under influence of LH, corpus luteum secretes increased progesterone which maintains secretory activity of endometrium
if no embryo (no hCG from embryo targeting the corpus luteum) then corpus luteum will die and become corpus albicans.
b) Uterus
Endometrium is in secretory phase and ready for implantation of embryo
If no embryo then endometrium will return to menstrual phase.

D. Female Reproductive cycle hormone interaction a. Hypothalamus produces GnRH which causes release of FSH (and LH) from anterior.pituitary.

b. Increased FSH, at beginning of cycle, stimulates initiation of follicular growth

c. Increased estrogen from follicular cell proliferation causes positive feedback triggering LH peak (mid cycle).

d. LH peak triggers ovulation and leutinizing of burst mature follicle (corpus luteum)

e. Corpus luteum grows for next week producing increasing progesterone which acts to maintain endometrium so an implanting embryo can survive

f. Increased progesterone and estrogen causes inhibition of FH and FSH (therefore no more ovulation for the cycle)

g. Fate of oocyte

1) If not fertilized then decreasing LH causes corpus luteum to deteriorate and endometrium will be lost ( menses)

2) If fertilized then growing embryo produces Human Chorionic Gonadotropin (an embryonic LH) which causes the corpus luteum to keep making progesterone so endometrium stays during gestation (pregnancy).

On a single sheet of graph paper, diagram the changes of gonadotropin hormones, ovarian hormones, oocytes, follicular development, and phase of endometrium. Do this for a 28 day cycle.

 

Professor Thomas M. Lancraft

Human Anatomy and Physiology Courses 
at St. Petersburg College
St. Petersburg/Gibbs Campus

5/2008